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Specifications
- Antibody type:Primary
- Antigen name:interleukin 9
- Clonality:Polyclonal
- Gene ID:P15248
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:IL9
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:IL9
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-9 (human Interleukin-9).
- Purification:Anti-hIL-9 specific antibody was purified by affinity chromatography employing immobilized hIL-9 matrix.
- Cat. no.:10098-148
- Supplier No.:XP-5200
Specifications
About this item
IL-9 is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors; but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL-9 stimulates cell proliferation and prevents apoptosis. The IL-9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL-9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-9 (0.6 ng/mL), a concentration of 0.15 - 0.025 µg/mL of this antibody is required. ELISA: To detect hIL-9 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-9. Sandwich: To detect hIL-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-9. Western Blot: To detect hIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hIL-9 specific antibody was purified by affinity chromatography employing immobilized hIL-9 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: IL9
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human