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Specifications
- Antibody type:Primary
- Antigen name:interleukin 7
- Clonality:Polyclonal
- Gene ID:P10168
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Mouse
- Antigen symbol:Il7
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:Il7
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-7 (murine IL-7).
- Purification:Anti-mIL-7 specific antibody was purified by affinity chromatography employing immobilized mIL-7 matrix.
- Cat. no.:10098-140
- Supplier No.:XP-5198
Specifications
About this item
Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL), a concentration of 0.012 - 0.02 µg/mL of this antibody is required. ELISA: To detect mIL-7 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7. Sandwich: To detect mIL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-7 (XP-5198Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-7. Western Blot: To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-mIL-7 specific antibody was purified by affinity chromatography employing immobilized mIL-7 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Il7
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Mouse