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Specifications
- Antibody type:Primary
- Antigen name:interleukin 5 (colony-stimulating factor, eosinophil)
- Clonality:Polyclonal
- Gene ID:P05113
- Host:Rabbit
- Reactivity:Human
- Antigen symbol:IL5
- Conjugation:Biotin
- ELISA:Yes
- Size:0.05 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:IL5
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human IL-5).
- Purification:Anti-hIL-5 specific antibody was purified by affinity chromatography and then biotinylated.
- Cat. no.:10098-104
- Supplier No.:XP-5191BT
Specifications
About this item
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70% amino acid sequence homology.
ELISA: To detect hIL-5 by direct ELISA (using 100 µL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-5. Sandwich: To detect hIL-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-5 (XP-5191) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hIL-5 specific antibody was purified by affinity chromatography and then biotinylated.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: IL5
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human