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Specifications
- Antibody type:Primary
- Antigen name:interleukin 3 (colony-stimulating factor, multiple)
- Clonality:Polyclonal
- Gene ID:P08700
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:IL3
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:IL3
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human Interleukin-3).
- Purification:Anti-hIL-3 specific antibody was purified by affinity chromatography employing immobilized hIL-3 matrix.
- Cat. no.:10098-548
- Supplier No.:XP-5185
Specifications
About this item
The pleiotropic IL-3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (1.0 ng/mL), a concentration of 0.38 - 0.57 µg/mL of this antibody is required. ELISA: To detect hIL-3 by indirect ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3. Sandwich To detect hIL-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-3 (XP-5185Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3. Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hIL-3 specific antibody was purified by affinity chromatography employing immobilized hIL-3 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: IL3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human