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Specifications
- Antibody type:Primary
- Antigen name:interleukin 16
- Clonality:Polyclonal
- Gene ID:Q9UME6
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:IL16
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:IL16
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-16 (human Interleukin-16).
- Purification:Anti-hIL-16 specific antibody was purified by affinity chromatography employing immobilized hIL-16 matrix.
- Cat. no.:10098-538
- Supplier No.:XP-5170
Specifications
About this item
IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.20 ng/mL), a concentration of 0.12 - 0.15 µg/mL of this antibody is required. ELISA: To detect hIL-16 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16. Sandwich: To detect hIL-16 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hIL-16 specific antibody was purified by affinity chromatography employing immobilized hIL-16 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: IL16
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human