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Specifications
- Antibody type:Primary
- Antigen name:fms-related tyrosine kinase 3 ligand
- Clonality:Polyclonal
- Gene ID:P49771
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:FLT3LG
- Conjugation:Biotin
- ELISA:Yes
- Size:0.05 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:FLT3LG
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt3-Ligand.
- Purification:Anti-hFlt3-Ligand specific antibody was purified by affinity chromatography and then biotinylated.
- Cat. no.:10098-492
- Supplier No.:XP-5138BT
Specifications
About this item
Flt3-Ligand is a growth factor important for the proliferation of hematopoietic cells. Flt3-Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). Flt3-Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt3-Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/mL), a concentration of 4.0-6.0 ng/mL of this antibody is required.ELISA: To detect hFlt3-Ligand by direct ELISA (using 100 µL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFlt3-Ligand. Sandwich: To detect hFlt-3 Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Flt-3 Ligand (XP-5138Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand. Western Blot: To detect hFlt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hFlt3-Ligand specific antibody was purified by affinity chromatography and then biotinylated.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: FLT3LG
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human