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Specifications
- Antibody type:Primary
- Antigen name:fms-related tyrosine kinase 3 ligand
- Clonality:Polyclonal
- Gene ID:P49771
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:FLT3LG
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:FLT3LG
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hflt3-Ligand.
- Purification:Anti-hflt3-Ligand specific antibody was purified by affinity chromatography employing immobilized hflt3-Ligand matrix.
- Cat. no.:10098-482
- Supplier No.:XP-5138
Specifications
About this item
Flt3-Ligand is a growth factor important for the proliferation of hematopoietic cells. Flt3-Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). Flt3-Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt3-Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hflt3- Ligand (15 ng/mL), a concentration of 0.25 - 0.75 µg/mL of this antibody is required. ELISA: To detect hflt3-Ligand by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hflt 3-Ligand. Sandwich: To detect hFGF-basic by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-basic (XP-5138Bt) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic. Western Blot: To detect hflt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hflt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hflt3-Ligand specific antibody was purified by affinity chromatography employing immobilized hflt3-Ligand matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: FLT3LG
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human