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Specifications
- Antibody type:Primary
- Antigen name:Fas ligand (TNF superfamily, member 6)
- Clonality:Polyclonal
- Gene ID:P48023
- Host:Goat
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:FASLG
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:FASLG
- Shipping temperature:RT
- Immunogen:Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL (human FAS Ligand).
- Purification:Anti-hFasL specific antibody was purified by affinity chromatography employing immobilized hFasL matrix.
- Cat. no.:10098-454
- Supplier No.:XP-5130
Specifications
About this item
Fas Ligand is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of FasL/Apo1L (10 ng/mL), a concentration of 0.08 - 0.1 µg/mL of this antibody is required. ELISA: To detect hFasL by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFasL. Western Blot: To detect hFasL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hFasL specific antibody was purified by affinity chromatography employing immobilized hFasL matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: FASLG
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Human