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Specifications
- Antibody type:Primary
- Antigen name:phosphatase and tensin homolog
- Clonality:Polyclonal
- Gene ID:P60484
- Host:Rabbit
- Reactivity:Human,Rat,Mouse
- Antigen symbol:PTEN
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:PTEN
- Storage buffer:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Molecular weight:54 kDa
- Storage temperature:Store antibody at –20 °C for up to one year.
- Concentration:1 mg/mL
- Shipping temperature:4 °C
- Immunogen:PTEN (Ab-370) antibody was raised against a peptide sequence around aa.368~372 (D-V-S-D-N) derived from Human PTEN.
- Purification:Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- Cat. no.:10068-814
- Supplier No.:79-297
Specifications
About this item
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate, phosphatidylinositol 3, 4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1, 3, 4, 5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3, 4, 5)P3 > PtdIns(3, 4)P2 > PtdIns3P > Ins(1, 3, 4, 5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: PTEN
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat