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Specifications
- Antibody type:Primary
- Antigen name:tubulin, alpha 3c
- Clonality:Polyclonal
- Gene ID:Q13748
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Antigen symbol:TUBA3C
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:TUBA3C
- Storage buffer:Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.
- Molecular weight:Predicted: 50 kDa Observed: 45 kDa
- Storage temperature:Alpha-tubulin antibody can be stored at 4 °C for three months and –20 °C, stable for up to one year.
- Concentration:1 mg/mL
- Shipping temperature:4 °C
- Immunogen:Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin. The immunogen is located within the first 50 amino acids of Alpha-tubulin.
- Purification:Alpha-tubulin antibody is affinity chromatography purified via peptide column.
- Cat. no.:10062-028
- Supplier No.:7599
Specifications
About this item
Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 ?g/ml. Antibody can also be used for Immunohistochemistry starting at 5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
Alpha-tubulin antibody is affinity chromatography purified via peptide column.
Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: TUBA3C
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat