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Specifications
- Antibody type:Primary
- Antigen name:Duffy blood group, atypical chemokine receptor
- Clonality:Polyclonal
- Gene ID:Q16570
- Host:Goat
- Reactivity:Human
- Antigen symbol:DARC
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:DARC
- Storage buffer:Duffy antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
- Storage temperature:Aliquot and store at –20 °C. Minimize freezing and thawing.
- Concentration:500 ug/mL
- Shipping temperature:4 °C
- Immunogen:Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.
- Tested applications:Fluorescence-Activated Cell Sorting
- Purification:Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Cat. no.:10112-500
- Supplier No.:45-502
Specifications
About this item
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 35 kDa band observed in human kidney and human liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 ?g/mL. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn lung showing endothelial staining. Recommended concentration, 3 ?g/mL. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
Duffy antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: DARC
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Human