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Specifications
- Antibody type:Primary
- Antigen name:amphiphysin
- Clonality:Polyclonal
- Gene ID:P49418
- Host:Goat
- Reactivity:Human,Mouse
- Antigen symbol:AMPH
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:AMPH
- Storage buffer:AmpH ipH ysin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
- Storage temperature:Aliquot and store at –20 °C. Minimize freezing and thawing.
- Concentration:500 ug/mL
- Shipping temperature:4 °C
- Immunogen:Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.
- Purification:Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Cat. no.:10115-310
- Supplier No.:45-006
Specifications
About this item
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8 (10) :2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5 ?g/mL. A minor band of unknown identity was also consistently observed at 45 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 ?g/mL.
AmpH ipH ysin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: AMPH
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Human, Mouse