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Specifications
- Antibody type:Primary
- Antigen name:serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
- Clonality:Polyclonal
- Gene ID:P05121
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:SERPINE1
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:SERPINE1
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1 (human Plasminogen Activator Inhibitor-1). Human PAI-1 specific antibody was purified by affinity chromatography employing immobilized hPAI-1 matrix.
- Cat. no.:10098-364
- Supplier No.:38-191
Specifications
About this item
PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type palsminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hPAI-1 (4.5 ?g/mL), a concentration of at least 15.0 ?g/mL of this antibody is required.
ELISA:
Indirect:
To detect hPAI-1 by indirect ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1.
Sandwich
To detect hPAI-1 by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human PAI-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1.
Western Blot:
To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
Type: Primary
Antigen: SERPINE1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human