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Specifications
- Antibody type:Primary
- Antigen name:serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
- Clonality:Polyclonal
- Gene ID:Q8IW75
- Host:Rabbit
- Reactivity:Human
- Antigen symbol:SERPINA12
- Conjugation:Biotin
- ELISA:Yes
- Size:0.05 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:SERPINA12
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography and then biotinylated.
- Cat. no.:10098-350
- Supplier No.:38-184
Specifications
About this item
Vaspin (serpina12) is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta sheets, nine alpha helices, and one central loop, which are distinctive structural features of Serpin family members. The
ELISA:
Direct:
To detect hVaspin by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Sandwich
To detect hVaspin by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Vaspin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Western Blot:
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
Type: Primary
Antigen: SERPINA12
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human