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Specifications
- Antibody type:Primary
- Antigen name:tumor necrosis factor receptor superfamily, member 1B
- Clonality:Polyclonal
- Gene ID:P20333
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:TNFRSF1B
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:TNFRSF1B
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor II. Human sTNF-receptor II specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor II matrix.
- Cat. no.:10097-904
- Supplier No.:38-123
Specifications
About this item
TNFRII (TNF Receptor II) is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF alpha but manifests a lower affinity to TNF beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNFRII consists of a 240 amino acid residue extracellular ligand binding portion, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human sTNF Receptor-II (0.5 ?g/mL) a concentration of 15.0 - 20.0 ?g/mL of this antibody is required.
ELISA:
Indirect:
To detect hsTNF-receptor II by indirect ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Sandwich
To detect hsTNF-receptor II by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human sTNF-receptor II as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Western Blot:
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
Type: Primary
Antigen: TNFRSF1B
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human