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Specifications
- Antibody type:Primary
- Antigen name:lymphocyte-specific protein tyrosine kinase
- Clonality:Monoclonal
- Clone:8E5F9 / 4B2C7
- Gene ID:P06239
- Host:Mouse
- Isotype:IgG2a
- Reactivity:Human
- Antigen symbol:LCK
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mL
- Western blot:Yes
- Antigen synonyms:LCK
- Storage buffer:Ascitic fluid containing 0.03% sodium azide.
- Storage temperature:Lck monoclonal antibody can be stored at –20 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Shipping temperature:4 °C
- Immunogen:Ni-NTA purified truncated recombinant Lck expressed in E. Coli strain BL21 (DE3).
- Cat. no.:10070-612
- Supplier No.:32-186
Specifications
About this item
Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Ascitic fluid containing 0.03% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: LCK
Clonality: Monoclonal
Clone: 8E5F9 / 4B2C7
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human