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Specifications
- Antibody type:Primary
- Antigen name:hypoxanthine phosphoribosyltransferase 1
- Clonality:Monoclonal
- Clone:1F8D11
- Gene ID:P00492
- Host:Mouse
- Isotype:IgG2b
- Reactivity:Human
- Antigen symbol:HPRT1
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mL
- Western blot:Yes
- Antigen synonyms:HPRT1
- Storage temperature:HPRT monoclonal antibody can be stored at –20 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Shipping temperature:4 °C
- Immunogen:Ni-NTA purified truncated recombinant HPRT expressed in E. Coli strain BL21 (DE3).
- Cat. no.:10070-586
- Supplier No.:32-170
Specifications
About this item
The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA.The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism.This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy. This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.
Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: HPRT1
Clonality: Monoclonal
Clone: 1F8D11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human