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Specifications
- Antibody type:Primary
- Antigen name:B lymphoid tyrosine kinase
- Clonality:Monoclonal
- Clone:9D10D1
- Gene ID:P51451
- Host:Mouse
- Isotype:IgG2a
- Reactivity:Human
- Antigen symbol:BLK
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- Size:0.1 mL
- Western blot:Yes
- Antigen synonyms:BLK
- Storage buffer:Ascitic fluid containing 0.03% sodium azide.
- Storage temperature:BLK monoclonal antibody can be stored at –20 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Shipping temperature:4 °C
- Immunogen:Ni-NTA purified truncated recombinant BLK expressed in E. Coli strain BL21 (DE3).
- Cat. no.:10073-180
- Supplier No.:32-116
Specifications
About this item
BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking.Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR.
Western Blot:1:500 - 1:2,000
IHC(P)1:500 - 1:2,000
IHC(F)1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Ascitic fluid containing 0.03% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: BLK
Clonality: Monoclonal
Clone: 9D10D1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human