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Specifications
- Antibody type:Primary
- Antigen name:enoyl CoA hydratase 1, peroxisomal
- Clonality:Polyclonal
- Gene ID:Q13011
- Host:Rabbit
- Reactivity:Human,Rat,Mouse
- Antigen symbol:ECH1
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 ul
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:ECH1
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:36 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store ECH1 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ECH1.
- Purification:Antibody is purified by protein A chromatography method.
- Cat. no.:10109-146
- Supplier No.:29-885
Specifications
About this item
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The protein contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway.This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
ECH1 antibody can be used for detection of ECH1 by ELISA at 1:1562500. ECH1 antibody can be used for detection of ECH1 by western blot at 2.5 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by protein A chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: ECH1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat