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Specifications
- Antibody type:Primary
- Antigen name:nuclear transcription factor Y, beta
- Clonality:Polyclonal
- Gene ID:P25208
- Host:Rabbit
- Reactivity:Human,Dog,Rat,Mouse
- Antigen symbol:NFYB
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 ul
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:NFYB
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:23 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYB antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYB.
- Purification:Antibody is purified by peptide affinity chromatography method.
- Cat. no.:10107-326
- Supplier No.:28-933
Specifications
About this item
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.
NFYB antibody can be used for detection of NFYB by ELISA at 1:312500. NFYB antibody can be used for detection of NFYB by western blot at 0.5 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: NFYB
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat, Dog