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Specifications
- Antibody type:Primary
- Antigen name:DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
- Clonality:Polyclonal
- Gene ID:Q9BQ39
- Host:Rabbit
- Reactivity:Human,Dog
- Antigen symbol:DDX50
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 ul
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:DDX50
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:81 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDX50 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX50.
- Purification:Antibody is purified by protein A chromatography method.
- Cat. no.:10106-390
- Supplier No.:28-504
Specifications
About this item
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX50 is a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. DDX50 and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. DDX50 gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.
DDX50 antibody can be used for detection of DDX50 by ELISA at 1:62500. DDX50 antibody can be used for detection of DDX50 by western blot at 2.5 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by protein A chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: DDX50
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Dog