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Specifications
- Antibody type:Primary
- Antigen name:nuclear transcription factor Y, alpha
- Clonality:Polyclonal
- Gene ID:P23511
- Host:Rabbit
- Reactivity:Human,Dog,Rat,Mouse
- Antigen symbol:NFYA
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- Size:100 ul
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:NFYA
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:37 kDa, 34 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYA antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYA.
- Purification:Antibody is purified by peptide affinity chromatography method.
- Cat. no.:10104-762
- Supplier No.:27-329
Specifications
About this item
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
NFYA antibody can be used for detection of NFYA by ELISA at 1:62500. NFYA antibody can be used for detection of NFYA by western blot at 0.2-2.0 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: NFYA
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat, Dog