An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD)

Chitin beads have been prepared having a magnetite core. This permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants; after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.