IMAC Sepharose™ High Performance Affinity Chromatography Media, Cytiva

Catalog #: CA95055-956L

Supplier: Cytiva

CAS Number:

SDS

IMAC Sepharose™ High Performance Affinity Chromatography Media, Cytiva

Catalog #: CA95055-956L

Supplier: Cytiva

CAS Number:

SDS

Specifications

Particle size:
34 µm
pH range:
3 - 12
Description:
IMAC Sepharose™ High Performance
Flow rate:
<300 cm/h
Size:
100 mL
Matrix:
Highly cross-linked agarose, 6%
Storage temperature:
4 - 30 °C
Binding capacity:
At least 40 mg histidine-tagged protein/mL medium when changed with Ni²⁺
Cat. no.:
CA95055-956L
Type:
Liquid
Supplier No.:
17-0920-07

Specifications

About this item

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

For optimizing purification of histidine-tagged proteins when Ni2+ is not the best choice of metal ion
Conveniently charge with your metal of choice
34 μm bead size for high performance purification
High binding capacity
Stable in commonly used aqueous buffers
Avoid using chelating agents, e.g. EDTA, EGTA, citrate

Conveniently charge with your metal of choice. IMAC Sepharose FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu₂₊, Zn₂₊, Ni₂₊, or Co₂₊); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

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