About this item
Protein G Mag Sepharose simplifies enrichment of target proteins by immunoprecipitation techniques, protein antigen precipitation from solutions using antibodies, or pull-down applications as an in vitro technique to detect physical interactions.
- Visible and dense Sepharose based magnetic beads with protein G ligand making it easy to spot and collect bound target protein
- Non-adherent beads eliminate smearing effects and aggregate formation
- Can be used without detergents
- Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target proteins—only low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
Protein G Mag Sepharose combines enrichment methods with magnetic beads, for small-scale sample preparation. These magnetic beads are coated with Sepharose, a beaded-form of agarose, with protein G as the ligand due to its high affinity for the Fc region of IgG antibodies.
Two protocols are available for protein enrichment: the cross-link protocol, linking proteins together via chemical bonds between amino acids, and the classic protocol for protein interaction. Both are simple elution conditions optimized for electrophoresis and mass spectrometry (MS).
Compatible with MagRack 6 and MagRack Maxi separation tools for handling the beads in microcentrifuge tubes.
Specifications
- Catalog No:
- CA89129-076
- MPCA29489432
- CA89129-102
- Binding capacity:
- 13 mg human IgG/ml medium
- 13 mg human IgG/ml medium
- 13 mg human IgG/ml medium
- Ligand:
- Protein G
- Protein G
- Protein G
- Matrix:
- Paramagnetic, spherical, highly cross-linked agarose particles
- Paramagnetic, spherical, highly cross-linked agarose particles
- Paramagnetic, spherical, highly cross-linked agarose particles
- Operating temperature:
- Room temperature
- Room temperature
- Room temperature
- Particle size:
- 37 μm - 100 μm
- 37 μm - 100 μm
- 37 μm - 100 μm
- Solubility:
- Liquid and suspension; easily soluble in cold water and hot water
- Liquid and suspension; easily soluble in cold water and hot water
- Liquid and suspension; easily soluble in cold water and hot water
- Storage conditions:
- 4 to 8°C, 20% Ethanol, 20% medium slurry
- 4 to 8°C, 20% Ethanol, 20% medium slurry
- 4 to 8°C, 20% Ethanol, 20% medium slurry
Specifications
Related Information
Certificates
Search by Lot Number28944008_HAND BOOK.pdf 28944008_INSTRUCTIONS.pdf 28951379_HAND BOOK.pdf 28951379_INSTRUCTIONS.pdf
Manual
89129-076_17073521