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Specifications
- Size:100 Assays
- Storage conditions:–80 °C (Long Term)
- Application:Fluorescence Microscopy
- Cat. no.:89165-920
- Supplier No.:51028-K100
Specifications
About this item
The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining.
- Fluorescence emission is easily multiplexed with common fluorescent dyes and fluorescent proteins
- Highly resistant to photo-bleaching, concentration quenching and photo-conversion
- Stringently manufactured to control and eliminate non-specific assay artifacts
The GOLGI ID® Green assay kit has been specifically designed for use with RFP-expressing cell lines as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).
The kit is suitable for use with live or fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A blue nuclear counterstain, Hoechst 33342, is provided to highlight this organelle as well.
It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.