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Specifications
- Description:Allylamine-dUTP
- Size:2.5 µmoL
- Purity detail:Purified by ion-exchange chromatography.
- Purity:≥93% (HPLC)
- Concentration:50mM
- Formulation:Liquid. Solution in water.
- Supplier No.:42861
Specifications
About this item
Allylamine-2’-deoxyuridine-5’-triphosphate can replace TTP in reactions where it serves as a substrate for E. coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).
Allylamine-dUTP is incorporated into DNA by a variety of labeling reactions including nick translation, random primed DNA synthesis and 3’ end labeling reactions. Allylamine-labeled DNA can be efficiently conjugated to the active ester form of signal generating moieties such as fluorescent dyes, biotin and other haptens to produce labeled probes for hybridization/detection assays. The efficient incorporation of Allylamine-dUTP, in contrast to many dye-labeled nucleotides, provides for greater incorporation of signaling moieties and stronger signals.