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Specifications
- Size:5 mg
- Protein/peptide name:Mmp Substrate (Colorimetric)
- Purity:97%
- Cat. no.:89160-964
- Supplier No.:P125-0005
Specifications
About this item
Sensitive chromogenic MMP substrate
Chromogenic substrate for continuous spectrophotometric assay of most matrix metalloproteinases, and TACE (ADAM17). The MMP cleavage site peptide bond is replaced by a thioester bond in this peptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's Reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm.