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Specifications
- Size:96 Wells
- Storage conditions:–80 °C (Long Term)
- Application:Fluorescent Detection, HTS Activity Assay, Cell-based Assays
- Cat. no.:89156-524
- Supplier No.:AK556-0001
Specifications
About this item
A FLUOR DE LYS® fluorescent assay system
- Useful for inhibitor screening or characterizing enzyme kinetics
- Includes optimal substrate selected from a panel of acetylated sites in p53 and histones
The Fluor de Lys® SIRT2 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® SIRT2 Substrate, which comprises a unique peptide based on amino acids 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), is incubated with SIRT2. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer II produces a fluorophore.
The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.