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Specifications
- Size:200 tests
- Storage conditions:–20 °C (Long Term)
- Application:Chemiluminescence
- Cat. no.:89156-018
- Supplier No.:850-248-KI01
Specifications
About this item
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
The assay utilizes bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis (TUNEL-based techniques and caspase assays), necrosis, growth arrest and cell proliferation simultaneously in mammalian cells
This highly sensitive assay (detects 100 mammalian cells/well) can be fully automated for high throughput measurements.
The assay utilizes the enzyme luciferase to catalyze the formation of light fom ATP and luciferin. The light can be measured using a luminometer or beta counter. ADP levels are measured by its conversion to ATP that is subsequently detected using the same reaction.