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Specifications
- Antibody type:Primary
- Antigen name:Microtubule-associated Protein 1 light chain 3 beta
- Clonality:Monoclonal
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:LC3B
- ImmunoChemistry:Yes
- Size:100 µg
- Western blot:Yes
- Form:Lyophilized from 1ml PBS containing PEG, sucrose, and 0.09% sodium azide.
- Antigen synonyms:MAP1LC3B|Autophagy-related protein LC3 B|MAP1 light chain 3-like protein 2|MAP1ALC3|MAP1A/MAP1B LC3 B|Microtubule-associated proteins 1A/1B light chain 3B [Precursor]|MAP1A/1B light chain 3 B|Autophagy-related ubiquitin-like modifier LC3 B
- Purification:Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography
- Cat. no.:89153-434
- Supplier No.:803-080-C100
Specifications
About this item
Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
- For Immunocytochemistry, Western Blot
Recognizes human, mouse, rat, dog and hamster LC3B. Recognizes both forms of endogenous LC3B. Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.
Reconstitute with 1ml water (15 minutes at room temperature).
Type: Primary
Antigen: LC3B
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human