About this item
TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.
TrueBlot® technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP IgG reagents). TrueBlot® is the unique horseradish peroxidase conjugated anti-mouse/anti-goat/anti-rabbit IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from the user's own IP antibody. TrueBlot® preferentially detects the non reduced form of IgG over the reduced, SDS-denatured form of IgG.
Applications include studies examining post-translational modification (e.g. phosphorylation or acetylation) or protein-protein interactions.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Catalog No:
- CA89409-738
- CA89409-760
- CA89409-758
- CA89409-736
- CA89409-764
- CA89409-742
- CA89409-740
- CA89409-762
- CA89409-746
- CA89409-766
- CA89409-744
- CA89409-748
- CA89409-770
- CA89409-768
- CA89409-752
- CA89409-772
- CA89409-750
- CA89409-756
- CA89409-734
- CA89409-754
- Species reactivity:
- Goat
- Mouse
- Mouse
- Mouse
- Mouse
- Mouse
- Goat
- Rabbit
- Sheep
- Rabbit
- Sheep
- Mouse
- Rabbit
- Rabbit
Specifications
Related Information
Certificates
Search by Lot Number18-8814-31-Goat-IgG-TrueBlot-IP-Western-Blot-Protocol.pdf 18-8814-33-Goat-IgG-TrueBlot-IP-Western-Blot-Protocol.pdf 18-8816-31-Rabbit-IgG-TrueBlot-IP-Western-Blot-Protocol.pdf 18-8816-33-Rabbit-IgG-TrueBlot-IP-Western-Blot-Protocol.pdf 18-8817-30-Mouse-IgG-TrueBlot-IP-Western-Blot-Protocol.pdf
Datasheet
About TrueBlot
Videos
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