To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Antibody type:Primary
- Antigen name:Histone H3
- Clonality:Polyclonal
- Gene ID:3020
- Host:Rabbit
- Isotype:IgG
- Reactivity:Bovine
- Antigen symbol:H3
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:50 μl
- Western blot:Yes
- Antigen synonyms:H3FA|HIST1H3J|H3FJ|HIST1H3E|HIST1H3G|HIST1H3B|H3FI|HIST1H3D|HIST1H3C|HIST1H3I|HIST1H3F|H3T|HIST3H3|H3FH|MGC126886|H3FD|H3FF|HIST1H3H|H3FC|H3FB|H3FT|H3FL|H3FK|HIST1H3A
- Modification:phospho-Ser10
- Storage buffer:Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Immunogen:Synthetic peptide conjugated to KLH, corresponding to amino acids 7-20 of Human Histone H3, phosphorylated at S10.
- Tested applications:ICC/IF, WB
- Purification:IgG fraction
- Cat. no.:89359-238
- Supplier No.:GTX12182
Specifications
About this item
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Phosphorylation of histone H3, referred to as the nucleosomal response, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli.2, 3, 9-12 Phosphorylation of histone H3 on S10 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at this site is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos, and c-myc.
Indirect IF: Use at a dilution of 1/500 using human epitheloid carcinoma HeLa cell line treated with nocodazole. WB: Use at a dilution of 1/2000. Detects a band of approximately 17 kDa using whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: H3F3A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat, Xenopus laevis, Bovine