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Specifications
- Antibody type:Primary
- Antigen name:DNA (cytosine-5-)-methyltransferase 3 alpha
- Clonality:Monoclonal
- Clone:64B814
- Gene ID:13435
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:DNMT3A
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:2.1.1.37|DNA (cytosine-5)-methyltransferase 3A|DNA MTase HsaIIIA|M.HsaIIIA|Dnmt3a|DNA methyltransferase HsaIIIA
- Storage buffer:Preservative: 0.02% Sodium Azide; Constituents: PBS
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.5 mg/ml
- Immunogen:Bacteria expressed HIS-tag recombinant mouse Dnmt3a.
- Tested applications:IHC, WB
- Purification:Protein G purified
- Cat. no.:89367-210
- Supplier No.:GTX13887
Specifications
About this item
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt 1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
IHC: Use at an assay dependent dilution. WB: Use at a concentration of 2 ?g/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: DNMT3A
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human