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Specifications
- Antibody type:Primary
- Antigen name:Cytokeratin 18
- Clonality:Monoclonal
- Clone:DA-7
- Gene ID:3875
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:CK18
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:type I cytoskeletal 18|K18|CYK18|cytokeratin 18|keratin-18|cytokeratin-18|CK-18|cell proliferation-inducing protein 46|cell proliferation-inducing gene 46 protein|keratin|PIG46
- Storage buffer:Phosphate-buffered saline, pH 7.4 containing 15 mM sodium azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Human breast carcinoma cell line PMC-42
- Tested applications:ELISA, ICC/IF, IHC-P, IP, WB
- Purification:Protein A affinity purified
- Cat. no.:89360-874
- Supplier No.:GTX20669
Specifications
About this item
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
For ELISA: Use at an assay dependant dilution. For ICC: Use at an assay dependant dilution. For IHC-P: Use at a dilution of 1:10-1:100 For IP: Use at an assay dependant dilution. For WB: Use at a concentration of 1-2µg/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: CK18
Clonality: Monoclonal
Clone: DA-7
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: