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Specifications
- Antibody type:Primary
- Antigen name:3-fucosyl-N-acetyl-lactosamine
- Clonality:Monoclonal
- Clone:MEM-158
- Gene ID:2526
- Host:Mouse
- Isotype:IgM
- Reactivity:Human
- Antigen symbol:CD15
- Conjugation:FITC (Fluorescein Isothiocyanate)
- Flow cytometry:Yes
- Size:100 test
- Antigen synonyms:CD15|Granulocyte Associated Antigen|MC-480|BG-7|SSEA-1|SIG-3339-1000
- Storage buffer:Preservative: 15mM Sodium Azide. Constituents: PBS, BSA. pH 7.4
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Immunogen:Granulocyte cell preparation (Human).
- Tested applications:Fluorescence-Activated Cell Sorting
- Cat. no.:89360-444
- Supplier No.:GTX18272
Specifications
About this item
The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. CD15 antibodies recognize the tri saccharide 3 fucosyllactosamine (3Fl) which is present in lacto N fucopentaose III and in the blood group antigen X hapten. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.
FACS: Use at a concentration of 20 µl reagent/ 100µl of whole blood or 10^6 cells in a suspension. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CD15
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human