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Specifications
- Antibody type:Primary
- Antigen name:Matrix Metalloproteinase 24
- Clonality:Polyclonal
- Gene ID:17391
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human
- Antigen symbol:MMP24
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoPrecipitation:Yes
- Size:25μg
- Western blot:Yes
- Antigen synonyms:MMP25|MMP-24|MT-MMP 5|MT5MMP|MTMMP5|MT5-MMP|MT-MMP5
- Storage buffer:0.01M Phosphate-buffered saline, pH 7.4 containing 50% Glycerol and 0.1% Sodium azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Synthetic peptide corresponding to the cytoplasmic domain of mouse MMP24.
- Tested applications:Fluorescence-Activated Cell Sorting
- Purification:Immunogen affinity purified
- Cat. no.:89358-646
- Supplier No.:GTX11647
Specifications
About this item
Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2.
ELISA (indirect): Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. IHC (Frozen sections): Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:2,000. This dilution is determined using a concentrated cell culture media from a stimulated cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. The antibody identifies a band at 65 kD (zymogen) and also the activation and breakdown products. GTX11647 also recognizes non-reduced (native) MMP24. Note: Since cell types differ greatly in the quantity of MMP24 produced, the conditioned media may require mitogen stimulation to visualize the bands by Western blotting. Treatment of cells with concanavolin-A or the phorbol ester TPA stimulates production of MMP24 in some cell types, and the enzyme can be recovered in cell lysates. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: MMP24
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human