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32 results for Protein Extraction Reagents

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Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

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SMART Digest™ Protein Digests Kits

SMART Digest™ Protein Digests Kits

Supplier: Thermo Scientific

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.

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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Supplier: Thermo Scientific

Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.

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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

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BugBuster® HT Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E

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BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.

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BugBuster® Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.

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BugBuster® 10X Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.

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PopCulture® Reagent, MilliporeSigma

Supplier: MilliporeSigma

PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

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Lysonase™ Bioprocessing Reagent, EMD Millipore

Supplier: MilliporeSigma

Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.

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NP-40 Alternative, MilliporeSigma

Supplier: MilliporeSigma

NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.

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Lysing Matrix Z, MP Biomedicals

Lysing Matrix Z, MP Biomedicals

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Thermo Scientific

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Q-sep® QuEChERS Extraction Salts, Restek

Q-sep® QuEChERS Extraction Salts, Restek

Supplier: Restek

QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling

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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

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Pierce™ Detergent Removal Spin Column, Thermo Scientific

Pierce™ Detergent Removal Spin Column, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

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Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.

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Pierce™ Micrococcal Nuclease Solution, Thermo Scientific

Pierce™ Micrococcal Nuclease Solution, Thermo Scientific

Supplier: Thermo Scientific

The Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific

Supplier: Thermo Scientific

Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

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Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.

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Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Supplier: Thermo Scientific

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

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Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.

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Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific

Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

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Protein Reducing Agents, G-Biosciences

Protein Reducing Agents, G-Biosciences

Supplier: G-Biosciences

A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)

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