You searched for: Cloning Kits and Ligation
The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
E. coli DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.
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T4 DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
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NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E
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T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs
Supplier: New England Biolabs (NEB)
T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.
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Blunt/TA Ligase Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.
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NEBNEXT® ULTRA™ Ligation Module, New England Biolabs
Supplier: New England Biolabs (NEB)
The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.
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T4 RNA Ligase 2 Truncated, KQ, New England Biolabs
Supplier: New England Biolabs (NEB)
T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.
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RtcB Ligase
Supplier: New England Biolabs (NEB)
RtcB Ligase is expressed as a His-tagged fusion in E. coli.
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Taq DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA
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Thermostable 5' AppDNA/RNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum
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T7 DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.
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T3 DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.
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NEBUILDER HiFi DNA Assembly® Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.
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Gibson Assembly® Cloning Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.
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NEBridge Ligase Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.
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SPLINTR® Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.
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T4 RNA Ligase 2, New England Biolabs
Supplier: New England Biolabs (NEB)
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity
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Gibson Assembly Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
Gibson Assembly was developed by Dr
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NEBuilder HiFi DNA Assembly Bundle for Large Fragments, New England Biolabs
Supplier: New England Biolabs (NEB)
NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly
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Salt-T4 DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.
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T4 RNA Ligase 1 (ssRNA Ligase) (30,000 units/ml), New England Biolabs
Supplier: New England Biolabs (NEB)
T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)
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Supplier: Corning
$370.49 to $1,423.90