You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
1X TBS pH 7.8
Supplier: Rockland Immunochemical
1X Tris buffered saline (TBS) pH 7.8
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TBS 10X Buffer Solution, pH 7.4, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
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1X PBS Buffer with 1% Casein
Supplier: Rockland Immunochemical
Phosphate buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.
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20X SSC pH 7.0
Supplier: Rockland Immunochemical
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays).
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SSC, 20X Buffer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization
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IPG Buffers, Cytiva
Supplier: Cytiva
IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
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RNA Gel Loading Solution 10X, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989)
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10X PBS pH 7.2
Supplier: Rockland Immunochemical
10X Phosphate buffered saline (PBS) pH 7.2
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SSPE Buffer, 20X, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.
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20X SSC Buffer (Standard Sodium Citrate)
Supplier: GROWCELLS
Concentrated sodium chloride-sodium citrate buffer functions by providing high ionic strength to optimize the rate of probe annealing to target nucleic acid
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Blocking Solution, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.
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5X Inlet Buffers
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
5x Inlet Buffer, 10mL. Running buffer for use with the Femto Pulse system. 5 concentration.
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PBS Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
This PBS concentrate is a general all-purpose buffer specifically designed for use with the ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.
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DIGE Buffer Kit, Cytiva
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
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CRITERION™ Phosphate Buffer, pH 7.2
Supplier: HARDY DIAGNOSTICS CA
CRITERION™ Agar, bacteriological grade is used as a solidifying agent in the preparation of solid and semi-solid culture media.
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DNA Gel Loading Buffer, Omega Bio-Tek
Supplier: Omega Bio-Tek
The 50X TAE buffer is composed of 2M tris-acetate and 0.05M EDTA at pH 8.3
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Corning® Tris Base Buffer, Powder, Corning
Supplier: Corning
Tris is commonly used in buffer solutions in biochemical and molecular biological research
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4X SDS Sample Buffer, MilliporeSigma
Supplier: MilliporeSigma
The 4X SDS Sample Buffer is a standard formulation commonly used for SDS-PAGE analysis of proteins
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SSPE Buffer, 20X, Liquid Concentrate, OmniPur*.
Supplier: MilliporeSigma
Each liter of concentrate makes 20L of 1X SSPE (0.150M NaCl, 0.010M Sodium Phosphate, 0.001M EDTA).
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TBE Buffer Solution
Supplier: Biotium
5X TBE for preparation of agarose gel running buffer.
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TRIS-buffered saline (TBS, 10X) pH 7.4, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Electrophoresis Running Buffer
Supplier: Edvotek
Tris Acetate, pH 7.8, concentrated 50X (for 5 L—20 standard gels).
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TAE Buffer, Concentrate, OmniPur*. 25X.
Supplier: MilliporeSigma
Premixed powder
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TE Buffer, Concentrate, OmniPur*. 100X.
Supplier: MilliporeSigma
Colorless, hazefree liquid
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PBS, Phosphate Buffered Saline Tablets, MilliporeSigma
Supplier: MilliporeSigma
Dissolving one tablet in 1 liter of deionized H₂O yields 140 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4 at 25°C.
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PreMixed Running Buffers, IBI Scientific
Supplier: IBI Scientific
IBI provides a variety of premixed buffers in various configurations
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MOPS/SDS, 20X, Running Buffer, Teknova
Supplier: Teknova
MOPS/SDS running buffer is designed for protein electrophoresis.