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Toluene-α-sulfonyl fluoride 99%

Supplier: Thermo Scientific Chemicals

A powerful protease inhibitor

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Toluene-α-sulfonyl fluoride ≥99% (by GC-FID)

Supplier: Enzo Life Sciences

Irreversible inhibitor of serine proteases. Widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB1 receptor activity. Spleen membranes used for CB2 studies do not require this pretreatment, as FAAH activity is absent in these cells. Inhibits also cysteine proteases like papain (reversible by DTT treatment, Prod. No. ALX-280-001), as well as internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor see AEBSF.

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Toluene-α-sulfonyl fluoride

Supplier: Bachem Americas

Sequence: Phenylmethylsulfonyl fluoride
Synonym(s): Pms-F#PMSF

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Toluene-α-sulfonyl fluoride ≥99.0%, OmniPur®, Sigma-Aldrich®

Supplier: MilliporeSigma

White powder. Insoluble in water.

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Toluene-α-sulfonyl fluoride

Supplier: Thermo Scientific

Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

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Toluene-α-sulfonyl fluoride ≥98%, Calbiochem®

Supplier: MilliporeSigma

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. Effective concentration: 50µM.