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63 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs

PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs

Supplier: New England Biolabs (NEB)

The Ph.D.−7 phage display peptide library kit v2 is based on a combinatorial library of random heptapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Thermo Scientific

Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)

Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)

Supplier: ReVacc Scientific

This pseudotyped virus uses lentivirus packaging to carry full length HA, NA and M of Highly Pathogenic Avian Influenza (HPAI) A virus (A/Pelecanus/Peru/VFAR-140/2022(H5N1), clade 2.3.4.4b) (GenBank: OQ565628.1). The pseudotypes can be handled using BSL-2 containment practices.

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VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.5. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.12.1 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.12.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-COV-2 Omicron JN.1 Luciferase

VSV-Pseudovirus_SARS-COV-2 Omicron JN.1 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron JN.1. See mutation on our website. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/99 B1 strain)

VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/99 B1 strain)

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length fusion (F) protein of Human metapneumovirus (HMPV) isolate NL/1/99 B1 strain (GenBank: AY525843.1). HMPV is a respiratory virus with fusion (F) protein as the sole target of neutralizing antibodies. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.

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VSV-Pseudovirus_Sudan Ebola Luciferase, ReVacc Scientific

VSV-Pseudovirus_Sudan Ebola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Sudan ebolavirus strain (GenBank: KT878488.1).

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PH.D.™-12 Phage Display Peptide Library, New England Biolabs

PH.D.™-12 Phage Display Peptide Library, New England Biolabs

Supplier: New England Biolabs (NEB)

The Ph.D.™-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage

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Lambda DNA, New England Biolabs

Lambda DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)

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PhiX174 RF I DNA, New England Biolabs

PhiX174 RF I DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled).

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VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Zaire ebolavirus Makona strain (GenBank: KR781609.1).

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Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Thermo Scientific

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

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Dengue Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (Type 1)

Dengue Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (Type 1)

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of Dengue virus (DENV) (GenBank: AAB70694.1, SEROTYPE 1 strain) and a Renilla luciferase reporter. As the wild typed Dengue virus is handled under BSL-2 precautions, this Dengue virus RVP can be handled using BSL-2 containment practices.

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sparQ mRNA-Seq Kit

sparQ mRNA-Seq Kit

Supplier: Quantabio

Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.

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VSV-Pseudovirus_HCV H77 Strain Luciferase

VSV-Pseudovirus_HCV H77 Strain Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length E1E2 of Hepatitis C virus (HCV) (H77 strain) (GenBank: AAB67036.1).

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PH.D.™ Peptide Display Cloning System, New England Biolabs

PH.D.™ Peptide Display Cloning System, New England Biolabs

Supplier: New England Biolabs (NEB)

The Ph.D. Peptide ligands for a variety of targets can then be selected by the straightforward method of panning.

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M13K07 Helper Phage, New England Biolabs

M13K07 Helper Phage, New England Biolabs

Supplier: New England Biolabs (NEB)

M13KO7 is an M13 derivative which carries the mutation Met40Ile in gII, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication

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VSV-Pseudovirus_Ebola Bomali Strain Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Bombali Ebolavirus (GenBank: YP_009513277.1).

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Pierce™ Cell-Free Protein Expression Vectors, T7, Mammalian, Thermo Scientific

Pierce™ Cell-Free Protein Expression Vectors, T7, Mammalian, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the 1-Step Human in vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.

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West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of West Nile Virus (WNV) (GenBank: DQ211652.1, NY99 strain) and a Renilla luciferase reporter. As the wild typed WNV is handled under BSL-2 precautions, this WNV RVP can be handled using BSL-2 containment practices.

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PhiX174 RF II DNA, New England Biolabs

PhiX174 RF II DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form)

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Vsv-Pseudovirus Sars-Cov-2 Omicron Luciferase, ReVacc Scientific

Vsv-Pseudovirus Sars-Cov-2 Omicron Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron (B.1.1.529), and 18-aa cytoplasmic tail truncation for optimal infection.

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Pierce™ Gaussia Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Gaussia Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Thermo Scientific

Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Pierce™ Cypridina Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Cypridina Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Thermo Scientific

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca

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Pierce™ Green Renilla Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Green Renilla Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Thermo Scientific

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

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pUC19 Vector, New England Biolabs

pUC19 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pUC19 is a commonly used plasmid cloning vector in E.coli

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M13mp18 RF I DNA, New England Biolabs

M13mp18 RF I DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BF.7. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of ZIKV (GenBank: KU321639.1, SPH2015 strain) and a Renilla luciferase reporter.

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