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63 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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Ward's® Plasmid DNA for Transformations

Ward's® Plasmid DNA for Transformations

Supplier: Avantor

These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.

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Polyadenylic acid (poly-A)

Polyadenylic acid (poly-A)

Supplier: Cytiva

Polyadenylic acid (poly-A) is a single stranded RNA homopolymer.

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InterPlay Adenoviral TAP System, Agilent Technologies

InterPlay Adenoviral TAP System, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

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AAV Helper-Free System, Agilent Technologies

AAV Helper-Free System, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

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AdEasy Adenoviral Vector Systems, Agilent Technologies

AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.

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Expression vectors, pGEX

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

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pJY2 Plasmid, Enzo Life Sciences

pJY2 Plasmid, Enzo Life Sciences

Supplier: Enzo Life Sciences

pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.

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AdEasy Adenoviral Vector Systems, Agilent Technologies

AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

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pBlueScript II Vectors, Agilent Technologies

pBlueScript II Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

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Vector, E. coli BL21, Cytiva

Vector, E. coli BL21, Cytiva

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

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InterPlay Adenoviral TAP Systems, Agilent Technologies

InterPlay Adenoviral TAP Systems, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

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SureVector, Vectors, Agilent Technologies

SureVector, Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

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pBR322 from Escherichia coli, MP Biomedicals

Supplier: MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli.

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VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length fusion (F) protein of Human metapneumovirus (HMPV) isolate NL/1/00/A1 (294E). HMPV is a respiratory virus with fusion (F) protein as the sole target of neutralizing antibodies. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase

VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.75. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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M13mp18 Single-Stranded DNA, New England Biolabs

M13mp18 Single-Stranded DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

The single-stranded viral DNA is isolated from M13mp18

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pSNAPf Vector, New England Biolabs

pSNAPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells

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pBR322 Vector, New England Biolabs

pBR322 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pBR322 DNA is a commonly used plasmid cloning vector in E.coli.

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pTXB1 Vector DNA, New England Biolabs

pTXB1 Vector DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

pTXB1 is an E.coli expression vector in the IMPACT™ Kit.

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VSV-Pseudovirus_SARS-CoV-2 Alpha Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Alpha Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in variant of B.1.1.7 (Alpha strain), including: ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.4/5 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.4/5 (BA.4 and BA.5 variants share the same Spike mutations). The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-COV-2 Omicron XBB.1.16 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.16. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)

Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)

Supplier: ReVacc Scientific

This pseudotyped virus uses lentivirus packaging to carry full length HA, NA and M of Highly Pathogenic Avian Influenza (HPAI) A virus (A/Pelecanus/Peru/VFAR-140/2022(H5N1), clade 2.3.4.4b) (GenBank: OQ565628.1). The pseudotypes can be handled using BSL-2 containment practices.

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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Thermo Scientific

Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.5. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BQ.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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Ph.D.-12 Phage Display Peptide Library Kit v2, New England Biolabs

Ph.D.-12 Phage Display Peptide Library Kit v2, New England Biolabs

Supplier: New England Biolabs (NEB)

The Ph.D.−12 Phage display peptide library kit v2 is based on a combinatorial library of random dodecapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

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PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs

PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs

Supplier: New England Biolabs (NEB)

The Ph.D.−7 phage display peptide library kit v2 is based on a combinatorial library of random heptapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

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