GoTaq® Hot Start Master Mix is a premixed ready-to-use solution containing GoTaq® Hot Start Polymerase, dNTPs, MgCl₂ and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

• All-in-one master mix: Just add template and primers
• Eliminate nonspecific amplification with a hot start enzyme
• Green master mix for direct-to-gel analysis
• Use colourless master mix when post-amplification fluorescence or absorbance analysis is required
• Reactions can be set up in less than a minute; simply add your template, water and primers

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94 to 95 °C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 °C. GoTaq® Hot Start Polymerase exhibits 5´→3´exonuclease activity.

GoTaq® Hot Start Green Master Mix also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis.