- Cdt:96 Tests
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Hôte:
- Primary antibody reactivity:Human
- Cible:Complement C3
- Désignation:Human complement C3 ELISA kit
- Environmentally Preferable:
- Type déchantillon:Serum, plasma, tissue homogenates and other biological fluids
- Réactivité croisée:Human Complement C3 ELISA kit exhibits high specificity and excellent specificity for the detection of human Complement C3. No significant cross-reactivity or interference between Complement C3 and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 h 30 min
- Principe du test:Quantitative
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:7,813 - 500 ng/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:4,688 ng/ml
- Regulatory status:RUO
Human Complement C3 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Complement C3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA Kit
- Detection range: 7,813 to 500 ng/ml
- Sensitivity: 4,688 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human Complement C3 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Complement C3 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement C3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C3 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C3 Antibody, which binds the captured Complement C3 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C3 captured in each well. The concentration of Complement C3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.