Spécifications
- Cdt:96 Tests
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Hôte:
- Primary antibody reactivity:Mouse
- Cible:PCSK9
- Désignation:Mouse PCSK9 ELISA kit
- Environmentally Preferable:
- Type déchantillon:Serum, plasma, tissue homogenates and other biological fluids
- Réactivité croisée:Mouse PCSK9 ELISA kit exhibits high specificity and excellent specificity for the detection of mouse PCSK9. No significant cross-reactivity or interference between PCSK9 and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 h 30 min
- Principe du test:Quantitative
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:15,625 - 1000 pg/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:9,375 pg/ml
- Regulatory status:RUO
Spécifications
À propos de cet article
Mouse PCSK9 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse PCSK9 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample type: Serum, plasma, tissue homogenates and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse PCSK9 employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse PCSK9 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PCSK9 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PCSK9 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PCSK9 Antibody, which binds the captured PCSK9 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PCSK9 captured in each well. The concentration of PCSK9 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.