Specifications
- Cdt:96 Tests
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Hôte:
- Primary antibody reactivity:Rat
- Cible:Inhibin B
- Désignation:Rat inhibin B ELISA kit
- Environmentally Preferable:
- Type déchantillon:Serum, plasma, tissue homogenates and other biological fluids
- Réactivité croisée:Rat Inhibin B ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Inhibin B. No significant cross-reactivity or interference between Inhibin B and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 h 30 min
- Principe du test:Quantitative
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:15,625 - 1000 pg/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:9,375 pg/ml
- Regulatory status:RUO
Specifications
About this item
Rat Inhibin B ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Inhibin B in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Inhibin B ELISA kit (A79481) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Inhibin B in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Inhibin B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Inhibin B present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Inhibin B Antibody, which binds the captured Inhibin B present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Inhibin B captured in each well. The concentration of Inhibin B can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.