Co-immunoprecipitation (Co-IP) Kit, Pierce™
PIER26149
:
- Cdt:1 KIT
- Désignation:Co-immunoprecipitation (Co-IP) kit, sufficient for 50 reactions
- Seq:01
- Environmentally Preferable:
Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.
- Covalent attachment method and non-reducing elution system
- Covalent antibody immobilisation allows prepared IP resin to be reused
- Underivatised agarose beads can be used as negative control for non specific binding
- Co-IP method is compatible with any physiological protein sample buffer
Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting proteins (prey proteins). This kit covalently couples antibodies onto an amine-reactive resin.
: Kit includes AminoLink™ Plus coupling resin, 2 ml; coupling buffer (20X), 25 ml; sodium cyanoborohydride solution (5M), 0,5 ml; quenching buffer, 50 ml; wash solution, 60 ml; IP lysis/wash buffer, 2×50 ml; modified Dulbecco's PBS (20X), 25 ml; conditioning buffer (100X), 5 ml; elution buffer, 50 ml; lane marker sample buffer (5X), 5 ml; spin columns, screw cap, 50 columns; microcentrifuge collection tubes (100 tubes), 2 ml, 100 tubes; microcentrifuge collection tubes (50 tubes), 1,5 ml; and control agarose resin, 2 ml.