Immunomagnetic positive selection of particle-free human CD3+ cells.

• Highly purified human CD3+ cells isolated in less than 30 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™

Isolate highly purified and magnetic particle-free CD3+ cells from peripheral blood mononuclear cells or washed leukapheresis samples in as little as 30 minutes.

Using immunomagnetic positive selection, CD3+ cells are labeled with antibodies and magnetic particles, then separated without columns using an EasySep™ magnet. Unwanted cells are poured off, while desired cells remain in the tube. Bound magnetic particles are then removed from the isolated CD3+ cells, which are immediately available for downstream use. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands. The CD3 antigen is expressed on all T cells and CD56+ NKT cells.
For even higher efficiency, the process can be automated using RoboSep™.

This kit is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP. STEMCELL can work with you to qualify this reagent as an ancillary material under an approved investigational new drug.