pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.

• U6_F Primer can be used to sequence the target sequence after cloning
• Components: 1 vial of 5 ug lyophilized pGFP-C-shLenti shRNA cloning vector
• Stability: Vector DNA is stable for at least one year from date of receipt when stored at –20 °C